Validation study of 6 dye-based SF25™ PCR amplification kit

Ever since the inception of STR-based forensic DNA analysis for human identification and profiling, approach developed continuously. Most these developments are pertaining to achieve set best polymorphic loci analysis, high inhibitor tolerance capability, increase in sensitivity, reduction turnaround time, cost-effectiveness. On similar line, SF25™ PCR amplification kit, a 6 fluorescent dye-chemistry based autosomal STR kit was developed, which analyses 25 viz., FAM dye-labelled D3S1358, D13S317, D7S820, D16S539, D1S1656, Penta E; HEX TPOX, TH01, D2S1338, CSF1PO, D; SUM D19S433, vWA, D21S11, D18S51, D6S1043; LYN Amelogenin, D8S1179, D5S818, D12S391, FGA; PUR D22S1045, Y-indel; D2S441 D10S1248. The developmental validation Amplification reagents were carried out this study analyze its specificity, concordance. This multiplex showed higher sensitivity with 125 pg template generating complete profile (with 100% allele call). Out 316 samples, 32samples aberrant triallelic pattern/aberrant heterozygous pattern at D16S539 locus. possible discrepant homozygous condition locus D1S1656 might be due invasion alleles present adjacent Such resulted shortening allelic bin size ladder reveals range from 11 19.3 can interpreted according casework as deemed by end-users their samples.